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Title: method for gabapentin and methylcobalamin by hplc in combination
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Title: gas chromatography
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Gas Chromatography

A Quick Historical Perspective
¢ 1903 Adsorption chromatography
¢ 1952 Gas chromatography (GLC)
¢ 1968 High performance liquid chromatography (HPLC)



Gas Chromatography
HPLC

¢ Mobile phase changes
¢ Constant temperature
¢ Compounds partition from the mobile phase based on solubility.
¢ Elution is generally time or volume dependent


GC

¢ Mobile phase is constant
¢ Increasing temperature
¢ Compounds partition from the mobile ....etc

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Title: Chromatography
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1 Introduction
Chromatography is a powerful separation method that finds applications in all branch of science. Chromatography was invented and named by the Russian botanist Mikhail Tswett shortly after the turn of the last century. He employed this technique to separate various plant pigments such as chlorophylls and xanthophylls by passing solutions of these compounds through a glass column packed with finely divided calcium carbonate. The separated species appeared as colored bands on the column, which accounts fo ....etc

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Title: Super critical Fluids and its Applications
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Super critical Fluids and its Applications
INTRODUCTION

• Definition: Supercritical Fluid is any substance at a temperature and pressure above its Critical point.
• Example: Commonly used Supercritical fluids are CO2 & H2O.
Phase Diagram:
• Few SCF
PROPERTIES OF SCF
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Title: method for gabapentin and methylcobalamin by hplc in combination
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Title: Protein Characterization
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Protein Characterization
Why do we analyse proteins
?
• Proteins play crucial roles in nearly all biological processes. These many functions of proteins are a result of the folding of proteins into many distinct 3D structures.
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