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Gas Chromatography
A Quick Historical Perspective
¢ 1903 Adsorption chromatography
¢ 1952 Gas chromatography (GLC)
¢ 1968 High performance liquid chromatography (HPLC)



Gas Chromatography
HPLC
¢ Mobile phase changes
¢ Constant temperature
¢ Compounds partition from the mobile phase based on solubility.
¢ Elution is generally time or volume dependent


GC
¢ Mobile phase is constant
¢ Increasing temperature
¢ Compounds partition from the mobile ....etc

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1 Introduction
Chromatography is a powerful separation method that finds applications in all branch of science. Chromatography was invented and named by the Russian botanist Mikhail Tswett shortly after the turn of the last century. He employed this technique to separate various plant pigments such as chlorophylls and xanthophylls by passing solutions of these compounds through a glass column packed with finely divided calcium carbonate. The separated species appeared as colored bands on the column, which accounts fo ....etc

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Title: gel permeation chromatography
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