16-01-2012, 02:14 PM
Mining Microarray
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ORIGIN OF MICROARRAY
-Many experiments on molecular biology since 1970
-Development of DNA sequencing
-Polymerase Chain Reaction (PCR)
WHAT IS MICROARRAY ?
-Ordered set of DNA molecule of known sequence
-May consists of few hundreds to hundred of thousands
-Individual feature in the array at precisely definite location of the substrate
-Identity of the DNA molecule never changes
IMPORTANCE OF MICROARRAY
-They are small in size
-They can contain a large number of genes
-Revolutionary change in gene expression
-Identification of genes causing deadly diseases
DNA MICROARRAY
-Array means to place in an orderly or fixed way
-These are small, solid supports onto which the sequences from thousands of different genes are attached at fixed locations
-cDNA derived from mRNA is mostly used