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PRESENTED BY:
HARISH KUMAR.G


INTRODUCTION
• A vast range of molecular biology, cellular biology, and advanced validation tools are available in modern medical research today, and some of these tools, capable of separating proteins on gel then identifying them using lasers, have given rise –over the last 25 years – to a novel science called proteomics. Proteomics tools are broadly applied in human disease. They allow the classification of diseases on a molecular basis, giving deep insights into the path ....etc

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Why?
The number of found genes(ORFs) in human genome is much less than the number of expressed proteins, and therefore, more information is needed about the “working units” of the cells
General flow for proteomics analysis
Two-dimensional gel electrophoresis (2DE)
1st dimension
separation based on
the pI of proteins
• 2nd dimension
separation based on
the molecular weight
of proteins
• Several
visualization/detection
possibilities
Gel-based proteomics
• “Older”approach to screen ....etc

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ABSTARCT
Proteomics is biotechâ„¢s new new thing. It is basically concerned with the proteome, which is the collection of all the proteins in made by a personâ„¢s cells and tissues.
Proteomics seems to overcome the shortcomings of genomics. The enthusiasts of proteomics are racing to catalogue the proteins in our body to figure out how they network with one another. Proteomics can be broken down into three main activities: identifying all the proteins made in a given cell, tissue, or organism; determining how thos ....etc

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Proteomics is something new in the field of biotechnology. It is basically the study of the proteome, the collective body of proteins made y a person’s cells and tissues. Proteome research permits the discovery of new protein markers for diagnostic purposes and of novel molecular targets for drug discovery. It is obvious that without the help of instruments like mass spectrometers, electrophoresis equipments and x-ray crystallographs, proteomics would be impractical.The abundance of information provided by proteome research ....etc

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