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Presented By
Dr.A.G.Ingale
Associate professor
Department Of Biotechnology
(Sodium dodecyl Sulphate Polyacrylamide Gel Electrophoresis)
What is SDS-PAGE?
SDS (Sodium dodecyl Sulphate) is a detergent used to denature proteins and give them a negative charge
PAGE: Polyacrylamide Gel Electrophoresis
It is a technique to separate proteins by their molecular weight
What is in the Sample Buffer?
►Tris buffer to provide appropriate pH
►SDS (sodium dodecyl sulphate) detergent to dissolve proteins and give them a negative charge
►Glycerol to make samples sink into wells
►Bromophenol Blue dye to visualize samples
Why Use Acrylamide Gels to Separate Proteins?
Acrylamide gel: tight matrix
Ideal for protein separation
Smaller pore size than agarose
Proteins much smaller than intact
chromosonal DNA
average amino acid =
110 Dalton
Why do we need to denature the proteins?
For more information about this article,please follow the link:
http://davidson.edu/academic/biology/cou...SPAGE.html
