ABSTRACT
Amaranthus spinosus Linn. complete plant material was extracted successively by petroleum ether, chloroform, methanol, and water. All the extracts were subjected to in vitro non-enzymatic haemo-glycosylation method, i.e. an oxidation reaction. The degree of haemoglycosylation in the presence of different extracts of Amaranthus spinosus were measured colorimetrically at 520 nm. The preventive effect of haemoglobin glycosylation at the two concentration; 0.5 and 1 mg/mL was estimated as follows: pet. ether; 13.1%, 16.4%, chloroform; 5.7%, 12%, methanol: 36.91%, 56.07% and aqueous: 22.2%, 31.01 %, respectively. The α-tocopheral (Vitamin E) was used as standard.
Keywords: Amaranthus spinosus; chloroform; haemoglobin; haemoglycosylation; vitamin E
Bahan tumbuhan Amaranthus spinosus Linn. diekstrakkan secara berturut-turut dengan eter petroleum, kloroform, metanol dan air. Kesemua ekstrak telah ditindakkan secara in vitro dengan kaedah glikosilasi tanpa enzyme ke atas haemoglobin, satu tindak balas pengoksidaan. Darjah haemoglikosilasi dengan kehadiran ekstrak Amaranthus spinosus yang berlainan telah diukur secara kolorimetrik pada 520 nm. Kesan pencegahan ke atas glikosilasi haemoglobin pada dua kepekatan, 0.5 dan 1 mg/mL, telah dianggarkan seperti berikut: eter pet. 13.1% dan 16.4%; kloroform 5.7% dan 12%; metanol 36.91% dan 56.07%; dan akuas 22.2% dan 31.01% masing-masing. α-tokoferal (vitamin E) telah digunakan sebagai paiwai.
Kata kunci: Amaranthus spinosus; haemoglikosilasi; kloroform; haemoglobin; vitamin E
INTRODUCTION
Haemo glycosylation refers to the covalent bonding of blood glucose to the red blood cells. Normally, only a small percentage of blood glucose, usually between 4.5 and 6%, is covalently linked to the red blood cells in haemoglobin of the non diabetes population. Glycosylated haemoglobin is recommended for cheking blood sugar control in people who might be pre-diabetic and monitoring blood sugar control in patients with more elevated levels, termed diabetes mellitus. For a single blood sample, it provides far more revealing information on glycemic behavior than a fasting blood sugar value. That being said, fasting blood sugar tests are crucial in making treatment decision. The American diabetes association guidelines are similar to others in advising that the glycosylated haemoglobin test performed at least two times a year in patient with diabetes (American Diabetes Association 2007)

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