submitted by
kamal kant

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DNA Profiling
(DNA fingerprinting)
What is DNA Profiling?
A technique used by scientists to distinguish between individuals of the same species using only samples of their DNA
Who Invented it?
 The process of DNA fingerprinting was invented by Alec Jeffreys at the University of Leicester in 1985.
 He was knighted in 1994.
Stages of DNA Profiling
Stage 1:
Cells are broken down
to release DNA
If only a small amount of DNA is available it can be amplified using the polymerase chain reaction (PCR)
 Stages of DNA Profiling
Step 2:
The DNA is cut into fragments using restriction enzymes.
Each restriction enzyme cuts DNA at a specific base sequence.
 Stages of DNA Profiling
 The sections of DNA that are cut out are called restriction fragments.
 This yields thousands of restriction fragments of all different sizes because the base sequences being cut may be far apart (long fragment) or close together (short fragment).
 Stages of DNA Profiling
Stage 3:
 Fragments are separated on the basis of size using a process called gel electrophoresis.
 DNA fragments are injected into wells and an electric current is applied along the gel.
 Stages of DNA Profiling
DNA is negatively charged so it is attracted to the positive end of the gel.
The shorter DNA fragments move faster than the longer fragments.
DNA is separated on basis of size.
 Stages of DNA Profiling
 A radioactive material is added which combines with the DNA fragments to produce a fluorescent image.
 A photographic copy of the DNA bands is obtained.
 Stages of DNA Profiling
Stage 4:
 The pattern of fragment distribution is then analysed.
 Uses of DNA Profiling
 DNA profiling is used to solve crimes and medical problems
 Crime
 Forensic science is the use of scientific knowledge in legal situations.
 The DNA profile of each individual is highly specific.
 The chances of two people having exactly the same DNA profile is 30,000 million to 1 (except for identical twins).
Biological materials used for DNA profiling
 Blood
 Hair
 Saliva
 Semen
 Body tissue cells
DNA Profiling can solve crimes
 The pattern of the DNA profile is then compared with those of the victim and the suspect.
 If the profile matches the suspect it provides strong evidence that the suspect was present at the crime scene (NB:it does not prove they committed the crime).
 If the profile doesn’t match the suspect then that suspect may be eliminated from the enquiry.
 Example
 A violent murder occurred.
 The forensics team retrieved a blood sample from the crime scene.
 They prepared DNA profiles of the blood sample, the victim and a suspect as follows:
 Was the suspect at the crime scene?
Solving Medical Problems
DNA profiles can be used to determine whether a particular person is the parent of a child.
A childs paternity (father) and maternity(mother) can be determined.
This information can be used in
• Paternity suits
• Inheritance cases
• Immigration cases