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Now a day’s increasing in prices and depletion of fossil fuels, creates very necessary to find out an alternative fuel (biodiesel) from non-edible oil seeds. This paper deals with the Transesterification of non-edible seeds oil (Custard apple, Karanja and Mauha oil) to obtain Fatty Acid Methyl Ester (FAME) or biodiesel by means of methanol in presence of Potassium hydroxide catalyst at 65˚C. The properties of produced biodiesels and their blends for different ratios (B10, B20, B30, B50 and B100) are comparable with each other and ASTM biodiesel standards. The produced biodiesel which is having better properties among three is tested for their use as a substitute fuel for diesel engine. Tests have been conducted on engine for different blends of biodiesel with standard diesel, at an engine speed of 1500 rpm, fixed compression ratio 17.5, fixed injection pressure of 200bar and varying brake power. The performance parameters evaluated includes brake thermal efficiency, brake specific fuel consumption, exhaust gas temperature, Carbon monoxide (CO), carbon dioxide (CO2), Hydrocarbon (HC) and oxides of nitrogen (NOx) emissions against varying Brake Power (BP).