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The best optical microscopes can see structures in the 200 – 400 nm range, at their limits of resolution.
The STM can “see” structures on the order of 0.1 nm —another SPM, the AFM—can distinguish things on the order of 100 nm .
Optical microscopes are “diffraction limited”—the wavelength range of visible light (400-750 nm) sets the size of the smallest thing that can be imaged: ~ 400 nm.
Scanning Probe Microscopes don’t use visible light—they depend on measuring intermolecular ....etc

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Microscope Light Dimmer for Biomedical Image Enhancement with Virtual Instrumentation


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Title: Atomic force microscope Scanning electron microscope
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Atomic force microscopy
 It is also known as scanning force microscopy.
 AFM is a very high resolution type of scanning probe microscopy , with resolution of order of a fraction of nanometer.
 AFM is one of the foremost tools for imaging , measuring and manipulating matter at the nanoscale.
Modes of AFM
There are three modes in which AFM can be operated depending on application:-
1. Static mode (contact).
2. Dynamic mode(non contact).
3. Tapping mode.
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Title: scanning electron microscope full report
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SEM
Introduction

The optical microscope was invented in the early 17th century. Although this discovery that revolutionized science, these were the first microscopes little more than one type magnifying glasses. The centuries many scientists then looked for new versions of the microscope with an improved resolution. In the year 1900, the maximum resolution achievable with a light microscope reached: 2 micrometers.
This maximum resolution we can do as follows:
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Title: tool makers microscope wikipedia
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